rabbit antibodies against ho 1  (Bioss)

Journal: Frontiers in Microbiology

Article Title: Akkermansia muciniphila alleviates antibiotic- and LPS-induced oxidative stress via the p38α MAPK–Nrf2 signaling axis

doi: 10.3389/fmicb.2026.1753421

Figure Lengend Snippet: AKK alleviates ABX/LPS-induced pathological remodeling and inflammation in murine intestine via inhibition of the p38α MAPK pathway. (A) Schematic of the experimental design, illustrating the timeline of ABX/LPS challenge and the interventions with AKK or the p38α MAPK inhibitor (SB203580). (B) Body weight changes of mice in different treatment groups. (C) Disease activity index. The data points represent the daily measurements. To make the graph clear, the data at key time points are shown in the figure. (D) Representative images showing: (i) The gross appearance of the colon and cecum; (ii) H&E-stained sections of the ileum; and (iii) H&E-stained sections of the colon. (E) Quantitative analysis of colon length. (F) Histopathological colitis scores. (G–I) Morphometric analysis of the ileum, including villus height, crypt depth, and the villus height-to-crypt depth ratio (VCR). n = 6 independent biological replicates. All data are presented as mean ± SEM. # p < 0.05, ### p < 0.001, #### p < 0.0001 versus NC group; * p < 0.05, ** p > p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS + ABX/LPS group, + p < 0.05, ++ p < 0.01, +++ p < 0.01, +++ p < 0.001, +++p < 0.0001 versus the AKK-treated group.

Article Snippet: Antibodies against p-p38α MAPK (#4511), HO-1(#43966), NQO-1(#3187) were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques: Inhibition, Activity Assay, Staining

Journal: Frontiers in Microbiology

Article Title: Akkermansia muciniphila alleviates antibiotic- and LPS-induced oxidative stress via the p38α MAPK–Nrf2 signaling axis

doi: 10.3389/fmicb.2026.1753421

Figure Lengend Snippet: AKK directly inhibits phosphorylation and activation of p38α MAPK in human colonic epithelial cells. In the LPS-induced injury model of Ncm460 cells: (A) Identification of the optimal concentration and treatment time for AKK via CCK-8 assay. (B) AKK reverses the LPS-induced suppression of cell viability. Data are normalized to the control group (set as 1.0). (C) Representative western blot images of p38α MAPK and phosphorylated p38α MAPK (p-p38α MAPK). Blots for p38α MAPK and p-p38α MAPK were obtained from the same membrane. (D) Quantitative analysis of total p-p38α MAPK protein expression levels. n = 6 independent biological replicates. All data are presented as mean ± SEM. # p < 0.05, ### p < 0.001, #### p < 0.0001 versus p38KD group. * p < 0.05, ** p > p < 0.01, *** p < 0.001, **** p < 0.0001 versus LPS group.

Article Snippet: Antibodies against p-p38α MAPK (#4511), HO-1(#43966), NQO-1(#3187) were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques: Phospho-proteomics, Activation Assay, Concentration Assay, CCK-8 Assay, Control, Western Blot, Membrane, Expressing

Journal: Frontiers in Microbiology

Article Title: Akkermansia muciniphila alleviates antibiotic- and LPS-induced oxidative stress via the p38α MAPK–Nrf2 signaling axis

doi: 10.3389/fmicb.2026.1753421

Figure Lengend Snippet: Inhibition of p38α MAPK alleviates oxidative stress in human colonic epithelial cells by activating the Nrf2 pathway. In the LPS-induced injury model of Ncm460 cells: (A,B) Representative flow cytometry scatter plots and quantitative analysis of intracellular ROS levels. (C) Levels of cellular oxidative stress markers (GSH, SOD, MDA). (D) Representative western blot images of Nrf2 and its downstream targets HO-1 and NQO1. The representative western blot images presented here are accompanied by the corresponding internal control detected on the same membrane. (E–G) Quantitative analysis of protein and mRNA expression levels of Nrf2 (E) , HO-1 (F) , and NQO1 (G) . n = 6 independent biological replicates. All data are presented as mean ± SEM. # p < 0.05, ### p < 0.001, #### p < 0.0001 versus p38KD group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus LPS group.

Article Snippet: Antibodies against p-p38α MAPK (#4511), HO-1(#43966), NQO-1(#3187) were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques: Inhibition, Flow Cytometry, Western Blot, Control, Membrane, Expressing

Journal: Frontiers in Microbiology

Article Title: Akkermansia muciniphila alleviates antibiotic- and LPS-induced oxidative stress via the p38α MAPK–Nrf2 signaling axis

doi: 10.3389/fmicb.2026.1753421

Figure Lengend Snippet: AKK enhances migration and tight junction integrity in human colonic epithelial cells in a p38α MAPK-dependent manner. In the LPS-induced injury model of Ncm460 cells: (A) Representative images and quantitative analysis of wound closure rate in the scratch assay. (B) Representative western blot images of tight junction proteins. The representative western blot images presented here are accompanied by the corresponding internal control detected on the same membrane. (C–E) Quantitative analysis of protein and mRNA expression levels of ZO-1 (C) , Occludin (D) , and Claudin-1 (E) . n = 6 independent biological replicates. # p < 0.05, ### p < 0.001, #### p < 0.0001 versus p38KD group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus LPS group.

Article Snippet: Antibodies against p-p38α MAPK (#4511), HO-1(#43966), NQO-1(#3187) were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques: Migration, Wound Healing Assay, Western Blot, Control, Membrane, Expressing

Journal: Frontiers in Microbiology

Article Title: Akkermansia muciniphila alleviates antibiotic- and LPS-induced oxidative stress via the p38α MAPK–Nrf2 signaling axis

doi: 10.3389/fmicb.2026.1753421

Figure Lengend Snippet: Akk restores the intestinal barrier by upregulating MUC2 and tight junction expression in a p38α MAPK-dependent manner. (A) Modulation of the mucus barrier by Akk and the p38α MAPK inhibitor (SB203580): representative IHC images of MUC2 and quantitative analysis. (B) Representative western blot images of tight junction proteins across groups. The representative western blot images presented here are accompanied by the corresponding internal control detected on the same membrane. (C–E) Quantitative analysis of protein and mRNA expression levels of ZO-1 (C) , occludin (D) , and claudin-1 (E) . (F,G) Serum levels of intestinal barrier damage markers, DAO activity, and D-LA concentration. n = 6 independent biological replicates. All data are presented as mean ± SEM. # p < 0.05, ### p < 0.001, #### p < 0.0001 versus NC group; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS + ABX/LPS group, + p < 0.05, ++ p < 0.01, +++ p < 0.01, +++ p < 0.001, +++ p < 0.0001 versus the AKK-treated group.

Article Snippet: Antibodies against p-p38α MAPK (#4511), HO-1(#43966), NQO-1(#3187) were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques: Expressing, Western Blot, Control, Membrane, Activity Assay, Concentration Assay

Journal: Frontiers in Microbiology

Article Title: Akkermansia muciniphila alleviates antibiotic- and LPS-induced oxidative stress via the p38α MAPK–Nrf2 signaling axis

doi: 10.3389/fmicb.2026.1753421

Figure Lengend Snippet: AKK alleviates intestinal oxidative stress by activating the Nrf2 signaling pathway through inhibition of p38α MAPK. (A) Levels of oxidative stress markers in colonic tissue: GSH, SOD, and MDA (B) Representative western blot images of Nrf2 and its downstream targets, HO-1 and NQO1. The representative western blot images presented here are accompanied by the corresponding internal control detected on the same membrane. n = 6 independent biological replicates. (C–E) Quantitative analysis of protein and mRNA expression levels of Nrf2 (C) , HO-1 (D) , and NQO1 (E) . n = 6 independent biological replicates. All data are presented as mean ± SEM. # p < 0.05, ### p < 0.001, #### p < 0.0001 versus NC group; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS + ABX/LPS group, + p < 0.05, ++ p < 0.01, +++ p < 0.01, +++ p < 0.001, +++ p < 0.0001 versus the A-treated group.

Article Snippet: Antibodies against p-p38α MAPK (#4511), HO-1(#43966), NQO-1(#3187) were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques: Inhibition, Western Blot, Control, Membrane, Expressing

Journal: Frontiers in Microbiology

Article Title: Akkermansia muciniphila alleviates antibiotic- and LPS-induced oxidative stress via the p38α MAPK–Nrf2 signaling axis

doi: 10.3389/fmicb.2026.1753421

Figure Lengend Snippet: Molecular docking predicts direct binding of AKK to p38α MAPK and validation in a murine model. Exploratory molecular docking results demonstrating strong binding between AKK and p38α MAPK: (A) Detailed view of the binding mode, with a binding free energy of −7.0 kcal·mol −1 ; (B) 3D representation of binding site interactions (yellow dashed lines indicate hydrogen bonds); (C) Schematic 3D structure of the complex; (D) 2D diagram of binding site interactions. (E) Top ten ranked binding conformations from docking screening. (F–I) Experimental validation in an ABX/LPS-induced mouse intestinal injury model: (F) Representative western blot images of p38α MAPK and p-p38α MAPK in colonic tissue; Blots for p38α MAPK and p-p38α MAPK were obtained from the same membrane. (G) p38α MAPK mRNA expression levels in the colon; (H) p-p38α MAPK/p38α MAPK ratio; (I) Total p-p38α MAPK protein levels. n = 6 independent biological replicates. All data are presented as mean ± SEM. # p < 0.05, ### p < 0.001, #### p < 0.0001 versus NC group; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS + ABX/LPS group, + p < 0.05, ++ p < 0.01, +++ < 0.01, +++ p < 0.001, +++ p < 0.0001 versus the AKK-treated group.

Article Snippet: Antibodies against p-p38α MAPK (#4511), HO-1(#43966), NQO-1(#3187) were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques: Binding Assay, Biomarker Discovery, Western Blot, Membrane, Expressing

Journal: Frontiers in Microbiology

Article Title: Akkermansia muciniphila alleviates antibiotic- and LPS-induced oxidative stress via the p38α MAPK–Nrf2 signaling axis

doi: 10.3389/fmicb.2026.1753421

Figure Lengend Snippet: A schematic model illustrating the role of the AKK-p38α MAPK-Nrf2 axis and microbial regulation in restoring intestinal homeostasis.

Article Snippet: Antibodies against p-p38α MAPK (#4511), HO-1(#43966), NQO-1(#3187) were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques:

Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: Calprotectin Is a Circulating Biomarker and Potential Therapeutic Target for Sarcopenia in Chronic Obstructive Pulmonary Disease

doi: 10.1002/jcsm.70196

Figure Lengend Snippet: Paquinimod attenuates the inflammatory response and oxidative stress in the cigarette smoke‐exposed mouse model. (A) The mRNA levels of inflammatory factors (TNF‐α, IL‐6, IL‐8, CXCL1, and IL1β) in skeletal muscle measured by qPCR. (B) TNF‐α and IL‐6 levels in different groups measured by ELISAs. (C) Levels of GSH, MDA, SOD2, and T‐AOC in the mouse Gast muscle. (D) Western blot analysis of the levels of antioxidant markers (Nrf2 and HO‐1) in each group of mice. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: The primary antibodies used were a rabbit polyclonal antibody against GAPDH (1:1000; Cell Signalling Technology, 5174), a mouse monoclonal antibody against atrogin‐1 (1:1000; Santa Cruz Biotechnology, sc‐166 806), a mouse monoclonal antibody against MuRF1 (1:1000; Santa Cruz Biotechnology, sc‐398 608), a rabbit monoclonal antibody against Nrf2 (1:1000; Cell Signalling Technology, 12721), and a rabbit monoclonal antibody against HO‐1 (1:1000; Cell Signalling Technology, 26416).

Techniques: Western Blot

Journal: International Journal of Nanomedicine

Article Title: Antioxidant Carbon Dots and Ursolic Acid Co-Encapsulated Liposomes Composite Hydrogel for Alleviating Adhesion Formation and Enhancing Tendon Healing in Tendon Injury

doi: 10.2147/IJN.S466312

Figure Lengend Snippet: Preparation of RCDs/UA@Lipo-HAMA and schematic diagram of therapeutic mechanisms against adhesion. ( A ) Preparation of RCDs/UA@Lipo for dual-drug co-loading. ( B ) The mixture of RCDs/UA@Lipo and HAMA was precisely applied to the periphery of the sutured tendon model and photocured using 365 nm UV irradiation. This process solidified the HAMA, enabling a localized and sustained release of RCDs and UA. ( C ) RCDs/UA@Lipo-HAMA diminishes oxidative stress by activating Nrf2/HO-1, reducing CD68, and iNOS, and suppressing fibrosis proteins, thereby lessening inflammation and fibrosis, reducing tendon adhesion, and promoting healing.

Article Snippet: After permeabilized and blocked, the sections were incubated with rabbit anti-rat antibodies against Nrf2 (1:200; Cat# 80593-1-RR, Proteintech, USA), HO-1 (1:200; Cat#10701-1-AP, Proteintech, USA), CD68 (1:300; GB11067, Servicebio, China), and iNOS (1:200; Cat#18985-1-AP, Proteintech, USA) at 4°C overnight.

Techniques: Irradiation

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Chemical Evolution and Biological Evaluation of Natural Products for Efficient Therapy of Acute Lung Injury.

doi: 10.1002/advs.202305432

Figure Lengend Snippet: Figure 7. In vivo evaluation of compound 8b for ALI treatment. A) Wet/dry ratio. B) Total protein concentration in BALF. C) Number of white blood cells in BALF. D) MPO activity in lung tissues. E) The amount of TNF-𝛼in BALF. F) The amount of IL-1𝛽in BALF. G) The amount of IL-6 in BALF. H) ROS level in BALF. I) NO level in BALF. J) MDA level in lung tissues. K) SOD activity in lung tissues. L) 8b inhibited MAPK and NF-𝜅B phosphorylation in lung tissues. M) The protein levels of iNOS and COX2 were determined by Western blotting analysis. N) The protein level of Keap1 in lung tissues. O) The protein levels of Nrf2 and HO-1, GCLM were determined by Western blotting analysis. P) Representative images of lung H&E staining of Control, LPS, and 8b treatment groups. Data for (H–J) are normalized to respective controls. All data were presented as means ± SD (n = 6; * vs LPS, # vs Con, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; #### p < 0.0001; ns, no significant vs LPS).

Article Snippet: Antibodies against Keap1 (#8047S), HO-1 (#43966S), COX-2 (#12282S), iNOS (#13120S), p-p65 (#3033), p65 (#8242), p-p38 (#4511S), p38 (#8690S), p-ERK (#9101), ERK (#9102), p-JNK (#4668), JNK (#9258), Ubiquitin (#3936) and GAPDH (#2118) were purchased from Cell Signaling Technology (Massachusetts, USA).

Techniques: In Vivo, Protein Concentration, Activity Assay, Phospho-proteomics, Western Blot, Staining, Control

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Chemical Evolution and Biological Evaluation of Natural Products for Efficient Therapy of Acute Lung Injury.

doi: 10.1002/advs.202305432

Figure Lengend Snippet: Figure 8. 8b regulates Keap1/Nrf2 signaling pathway and alleviates oxidative stress and inflammation for ALI therapy.

Article Snippet: Antibodies against Keap1 (#8047S), HO-1 (#43966S), COX-2 (#12282S), iNOS (#13120S), p-p65 (#3033), p65 (#8242), p-p38 (#4511S), p38 (#8690S), p-ERK (#9101), ERK (#9102), p-JNK (#4668), JNK (#9258), Ubiquitin (#3936) and GAPDH (#2118) were purchased from Cell Signaling Technology (Massachusetts, USA).

Techniques: